Arterial Puncture and Modified Allen Test

Key Points

  • Radial artery is preferred for arterial blood sampling, and collateral circulation must be screened first.
  • Modified Allen test is positive when hand flushes in 5-15 seconds after ulnar release.
  • Pre-heparinized syringes and minimal air exposure are essential for accurate ABG results.

Equipment

  • Pre-heparinized arterial blood gas syringe and needle
  • PPE and skin-preparation supplies
  • Timing aid for modified Allen test interpretation
  • Post-puncture pressure materials and specimen transport setup

Procedure Steps

  1. Confirm need for arterial sampling and select radial artery as first-choice site when appropriate.
  2. Perform modified Allen test before radial puncture.
  3. Ask patient to clench fist (or close hand manually if needed).
  4. Occlude both radial and ulnar arteries until hand blanches.
  5. Release ulnar pressure only and observe reperfusion.
  6. Interpret test: positive if flush returns in 5-15 seconds; do not puncture radial artery if negative.
  7. If positive, proceed with arterial puncture using pre-heparinized syringe and minimize air exposure.
  8. Apply prolonged direct pressure after sampling and monitor bleeding, especially with anticoagulant use.
  9. Transport specimen promptly and ensure correct mixing/handling to avoid sampling errors.

Common Errors

  • Skipping modified Allen test before radial puncture unsafe sampling in poor collateral flow.
  • Inadequate post-puncture pressure hematoma or persistent bleeding risk.
  • Air contamination or delayed transport inaccurate ABG interpretation.
  • Needle redirection at unsafe angles increased nerve/tissue injury risk.